Claude, 21. April 2018, 17:16 Uhr
Warning: Use of undefined constant ri_rand_compare - assumed 'ri_rand_compare' (this will throw an Error in a future version of PHP) in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/random-image-widget/random_image.php on line 129 Warning: Use of undefined constant ri_rand_compare - assumed 'ri_rand_compare' (this will throw an Error in a future version of PHP) in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/random-image-widget/random_image.php on line 130 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 1384 Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 700 Warning: Invalid argument supplied for foreach() in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 707 Warning: preg_match_all(): Compilation failed: invalid range in character class at offset 4 in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 700 Warning: Invalid argument supplied for foreach() in /home/httpd/vhosts/scratchbook.ch/httpdocs/wp-content/plugins/lightbox-plus/classes/shd.class.php on line 707
Jedes Jahr landen fast acht Millionen Tonnen Plastik im Meer. Bis sich der Kunststoff unter diesen Bedingungen zersetzt hat, dauert es Ewigkeiten. Eine Plastikflasche braucht beispielsweise 450 Jahre, um komplett zu zerfallen. In Großbritannien setzten Forscher nun auf spezielle Bakterien, um die Umweltverschmutzung in den Griff zu bekommen.
Das neue Enzym zersetzt Plastik viel schneller
Der gefräßige Mikroorganismus ‚Ideonella Sakaiensis‘ ist der Forschung schon länger bekannt. Wissenschaftler entdeckten, dass die Bakterien sich auf Flaschen ansiedelten und das Plastik mithilfe eines Enzyms verdauten. Das Problem war bisher nur, dass es immer noch lange dauerte, bis das Plastik komplett zersetzt war. Für eine dünne Plastikfolie brauchten die Bakterien rund 60 Wochen.
Wissenschaftler experimentierten nun mit dem Enzym, das die Bakterien zum Verdauen des Plastiks brauchen. „Wir haben das Erbgut herausgenommen und mit dem Enzym herum-experimentiert und bei diesem Vorgang haben wir durch Zufall ein schnelleres Enzym entwickelt“, erklärte John McGeehan, Professor an der Universität Portsmouth. Die Bakterien schaffen es jetzt, in 96 Stunden eine komplette Plastikflasche zu zerlegen.
***
Engineering a plastic-eating enzyme
Scientists have engineered an enzyme which can digest some of our most commonly polluting plastics, providing a potential solution to one of the world’s biggest environmental problems.
The discovery could result in a recycling solution for millions of tonnes of plastic bottles, made of polyethylene terephthalate, or PET, which currently persists for hundreds of years in the environment.
The research was led by teams at the University of Portsmouth and the US Department of Energy’s National Renewable Energy Laboratory (NREL) and is published in Proceedings of the National Academy of Sciences (PNAS).
Professor John McGeehan at the University of Portsmouth and Dr Gregg Beckham at NREL solved the crystal structure of PETase—a recently discovered enzyme that digests PET— and used this 3D information to understand how it works. During this study, they inadvertently engineered an enzyme that is even better at degrading the plastic than the one that evolved in nature.
The researchers are now working on improving the enzyme further to allow it to be used industrially to break down plastics in a fraction of the time.
Professor McGeehan, Director of the Institute of Biological and Biomedical Sciences in the School of Biological Sciences at Portsmouth, said: “Few could have predicted that since plastics became popular in the 1960s huge plastic waste patches would be found floating in oceans, or washed up on once pristine beaches all over the world.
“We can all play a significant part in dealing with the plastic problem, but the scientific community who ultimately created these ‘wonder-materials’, must now use all the technology at their disposal to develop real solutions.”
The researchers made the breakthrough when they were examining the structure of a natural enzyme which is thought to have evolved in a waste recycling centre in Japan, allowing a bacterium to degrade plastic as a food source.
PET, patented as a plastic in the 1940s, has not existed in nature for very long, so the team set out to determine how the enzyme evolved and if it might be possible to improve it.
The goal was to determine its structure, but they ended up going a step further and accidentally engineered an enzyme which was even better at breaking down PET plastics.
“Serendipity often plays a significant role in fundamental scientific research and our discovery here is no exception,” Professor McGeehan said.
“Although the improvement is modest, this unanticipated discovery suggests that there is room to further improve these enzymes, moving us closer to a recycling solution for the ever-growing mountain of discarded plastics.”
The research team can now apply the tools of protein engineering and evolution to continue to improve it.
The University of Portsmouth and NREL collaborated with scientists at the Diamond Light Source in the United Kingdom, a synchrotron that uses intense beams of X-rays 10 billion times brighter than the sun to act as a microscope powerful enough to see individual atoms.
Using their latest laboratory, beamline I23, an ultra-high-resolution 3D model of the PETase enzyme was generated in exquisite detail.
Professor McGeehan said: “The Diamond Light Source recently created one of the most advanced X-ray beamlines in the world and having access to this facility allowed us to see the 3D atomic structure of PETase in incredible detail. Being able to see the inner workings of this biological catalyst provided us with the blueprints to engineer a faster and more efficient enzyme.”
Chief Executive of the Diamond Light Source, Professor Andrew Harrison, said: “With input from five institutions in three different countries, this research is a fine example of how international collaboration can help make significant scientific breakthroughs.
“The detail that the team were able to draw out from the results achieved on the I23 beamline at Diamond will be invaluable in looking to tailor the enzyme for use in large-scale industrial recycling processes. The impact of such an innovative solution to plastic waste would be global. It is fantastic that UK scientists and facilities are helping to lead the way.”
With help from the computational modeling scientists at the University of South Florida and the University of Campinas in Brazil, the team discovered that PETase looks very similar to a cutinase, but it has some unusual features including a more open active site, able to accommodate man-made rather than natural polymers. These differences indicated that PETase may have evolved in a PET-containing environment to enable the enzyme to degrade PET. To test that hypothesis, the researchers mutated the PETase active site to make it more like a cutinase.
And that was when the unexpected happened – the researchers found that the PETase mutant was better than the natural PETase in degrading PET.
Significantly, the enzyme can also degrade polyethylene furandicarboxylate, or PEF, a bio-based substitute for PET plastics that is being hailed as a replacement for glass beer bottles.
Professor McGeehan said: “The engineering process is much the same as for enzymes currently being used in bio-washing detergents and in the manufacture of biofuels – the technology exists and it’s well within the possibility that in the coming years we will see an industrially viable process to turn PET and potentially other substrates like PEF, PLA, and PBS, back into their original building blocks so that they can be sustainably recycled.”
The research was funded by the University of Portsmouth, NREL and the Biotechnology and Biological Sciences Research Council (BBSRC).
Dr Colin Miles, Head of Strategy for Industrial Biotechnology at BBSRC, said: “This is a highly novel piece of science based on a detailed molecular-level understanding of an enzyme able to depolymerise a common type of plastic, whose persistence in the environment has become a global issue. It will be interesting to see whether, based on this study, the performance of the enzyme can be improved and made suitable for industrial-scale application in the recycling and the future circular economy of plastic.”
The paper’s lead author is postgraduate student jointly funded by the University of Portsmouth and NREL, Harry Austin.
He said: “This research is just the beginning and there is much more to be done in this area. I am delighted to be part of an international team that is tackling one of the biggest problems facing our planet.”
Principal beamline scientist on I23 at Diamond, Dr Armin Wagner, said: “The long-wavelength macromolecular crystallography beamline I23 at Diamond is an incredibly advanced and unique facility that enables us to solve structures that are usually difficult to characterise. In the case of PETase, the protein crystals diffracted really well and we were able to achieve very high resolution. While most of the known protein structures have been determined to resolutions between 1.5 and 3.0 Å (0.15 – 0.3 nanometres), and we could achieve from for one of the investigated structures 0.92 Å. It basically makes what we are looking at much clearer and therefore easier to understand.
“The high resolution 3D structure allowed us to get a clear picture of where the enzyme grips its target which then informed the next step of computational modelling to investigate the mechanism of PET degradation further. The large, curved area detector in combination with the vacuum environment on the I23 beamline is ideal for this work as it allows high resolution structure determinations at low X-ray doses limiting detrimental radiation damage effects to the crystals.”
(Quelle)
